Wednesday 15 July 2020

Embryology

In coconut, the water inside the fruit is its free nuclear endosperm and its edible part is

cellular endosperm. Cellular part gradually gains thickness as the fruit attains maturity.
Free nuclear part of endosperm in coconut Is commonly called coconut milk. It is highly nutrient rich drink containing cytokinins or similar growth hormone to accelerate growth of callus in tissue culture medium. It contains polysaccharides, polypeptide and polynucleotides with essential minerals and vitamins. Coconut Oil is extracted from the cellular endosperm of coconut fruit.
Tissue Culture Tips-
Use of various concentrations and combinations of auxins and cytokinins for proper differentiations of root and shoot in callus (0.2 mg/L kinetine induces vigourous growth in callus, 3.0 mg/L IAA with 0.2 mg/L kinetine induces rooting in callus, 2-3 mg/L kinetine with 0.2 mg/L IAA induces shoot formation in callus).

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